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pcdna3 1 vector  (Addgene inc)


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    Structured Review

    Addgene inc pcdna3 1 vector
    Pcdna3 1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcdna3+1+flag+ha+vector/FLAG-HA-pcDNA3%2E1-+(Plasmid+%2352535)/10__1158_slash_0008___5472__can___25___2893-62-10-12
    Average 95 stars, based on 115 article reviews
    pcdna3 1 vector - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Identification of HnRNPC as a novel Tau exon 10 splicing factor using RNA antisense purification mass spectrometry.
    Article Snippet: .. Tau minigene (4431 bp) was obtained by PCR amplification of three fragments from the genomic DNA of HEK 293 FT cells between MAPT exons 9–11 (Figure 1(c)), which were then seamlessly cloned together to the pCDNA3.1-FLAG-HA vector (Addgene, USA) in one step by using GeneArt® Seamless Cloning and Assembly Enzyme Mix (Thermo Scientific, USA). .. Total RNA from cells was extracted using the RNeasy®mini kit (Qiagen, USA) according to the manufacturer’s protocol and were used for both semiquantitative and quantitative PCR.

    Article Title: Identification of HnRNPC as a novel Tau exon 10 splicing factor using RNA antisense purification mass spectrometry
    Article Snippet: .. Tau minigene (4431 bp) was obtained by PCR amplification of three fragments from the genomic DNA of HEK 293 FT cells between MAPT exons 9–11 ( )), which were then seamlessly cloned together to the pCDNA3.1-FLAG-HA vector (Addgene, USA) in one step by using GeneArt® Seamless Cloning and Assembly Enzyme Mix (Thermo Scientific, USA). .. Total RNA from cells was extracted using the RNeasy®mini kit (Qiagen, USA) according to the manufacturer’s protocol and were used for both semiquantitative and quantitative PCR.

    Amplification:

    Article Title: Identification of HnRNPC as a novel Tau exon 10 splicing factor using RNA antisense purification mass spectrometry.
    Article Snippet: .. Tau minigene (4431 bp) was obtained by PCR amplification of three fragments from the genomic DNA of HEK 293 FT cells between MAPT exons 9–11 (Figure 1(c)), which were then seamlessly cloned together to the pCDNA3.1-FLAG-HA vector (Addgene, USA) in one step by using GeneArt® Seamless Cloning and Assembly Enzyme Mix (Thermo Scientific, USA). .. Total RNA from cells was extracted using the RNeasy®mini kit (Qiagen, USA) according to the manufacturer’s protocol and were used for both semiquantitative and quantitative PCR.

    Article Title: cGAS-STING effectively restricts murine norovirus infection but antagonizes the antiviral action of N-terminus of RIG-I in mouse macrophages
    Article Snippet: .. The full-length mouse RIG-I gene and its N-terminus, and the N-terminus of mouse MDA5 were amplified from a cDNA sample that isolated from RAW264.7 cells and cloned into pcDNA3.1/Flag-HA vector (Addgene), respectively. ..

    Article Title: Identification of HnRNPC as a novel Tau exon 10 splicing factor using RNA antisense purification mass spectrometry
    Article Snippet: .. Tau minigene (4431 bp) was obtained by PCR amplification of three fragments from the genomic DNA of HEK 293 FT cells between MAPT exons 9–11 ( )), which were then seamlessly cloned together to the pCDNA3.1-FLAG-HA vector (Addgene, USA) in one step by using GeneArt® Seamless Cloning and Assembly Enzyme Mix (Thermo Scientific, USA). .. Total RNA from cells was extracted using the RNeasy®mini kit (Qiagen, USA) according to the manufacturer’s protocol and were used for both semiquantitative and quantitative PCR.

    Clone Assay:

    Article Title: Identification of HnRNPC as a novel Tau exon 10 splicing factor using RNA antisense purification mass spectrometry.
    Article Snippet: .. Tau minigene (4431 bp) was obtained by PCR amplification of three fragments from the genomic DNA of HEK 293 FT cells between MAPT exons 9–11 (Figure 1(c)), which were then seamlessly cloned together to the pCDNA3.1-FLAG-HA vector (Addgene, USA) in one step by using GeneArt® Seamless Cloning and Assembly Enzyme Mix (Thermo Scientific, USA). .. Total RNA from cells was extracted using the RNeasy®mini kit (Qiagen, USA) according to the manufacturer’s protocol and were used for both semiquantitative and quantitative PCR.

    Article Title: cGAS-STING effectively restricts murine norovirus infection but antagonizes the antiviral action of N-terminus of RIG-I in mouse macrophages
    Article Snippet: .. The full-length mouse RIG-I gene and its N-terminus, and the N-terminus of mouse MDA5 were amplified from a cDNA sample that isolated from RAW264.7 cells and cloned into pcDNA3.1/Flag-HA vector (Addgene), respectively. ..

    Article Title: Identification of HnRNPC as a novel Tau exon 10 splicing factor using RNA antisense purification mass spectrometry
    Article Snippet: .. Tau minigene (4431 bp) was obtained by PCR amplification of three fragments from the genomic DNA of HEK 293 FT cells between MAPT exons 9–11 ( )), which were then seamlessly cloned together to the pCDNA3.1-FLAG-HA vector (Addgene, USA) in one step by using GeneArt® Seamless Cloning and Assembly Enzyme Mix (Thermo Scientific, USA). .. Total RNA from cells was extracted using the RNeasy®mini kit (Qiagen, USA) according to the manufacturer’s protocol and were used for both semiquantitative and quantitative PCR.

    Cloning:

    Article Title: Identification of HnRNPC as a novel Tau exon 10 splicing factor using RNA antisense purification mass spectrometry.
    Article Snippet: .. Tau minigene (4431 bp) was obtained by PCR amplification of three fragments from the genomic DNA of HEK 293 FT cells between MAPT exons 9–11 (Figure 1(c)), which were then seamlessly cloned together to the pCDNA3.1-FLAG-HA vector (Addgene, USA) in one step by using GeneArt® Seamless Cloning and Assembly Enzyme Mix (Thermo Scientific, USA). .. Total RNA from cells was extracted using the RNeasy®mini kit (Qiagen, USA) according to the manufacturer’s protocol and were used for both semiquantitative and quantitative PCR.

    Article Title: Identification of HnRNPC as a novel Tau exon 10 splicing factor using RNA antisense purification mass spectrometry
    Article Snippet: .. Tau minigene (4431 bp) was obtained by PCR amplification of three fragments from the genomic DNA of HEK 293 FT cells between MAPT exons 9–11 ( )), which were then seamlessly cloned together to the pCDNA3.1-FLAG-HA vector (Addgene, USA) in one step by using GeneArt® Seamless Cloning and Assembly Enzyme Mix (Thermo Scientific, USA). .. Total RNA from cells was extracted using the RNeasy®mini kit (Qiagen, USA) according to the manufacturer’s protocol and were used for both semiquantitative and quantitative PCR.

    Isolation:

    Article Title: cGAS-STING effectively restricts murine norovirus infection but antagonizes the antiviral action of N-terminus of RIG-I in mouse macrophages
    Article Snippet: .. The full-length mouse RIG-I gene and its N-terminus, and the N-terminus of mouse MDA5 were amplified from a cDNA sample that isolated from RAW264.7 cells and cloned into pcDNA3.1/Flag-HA vector (Addgene), respectively. ..

    Plasmid Preparation:

    Article Title: cGAS-STING effectively restricts murine norovirus infection but antagonizes the antiviral action of N-terminus of RIG-I in mouse macrophages
    Article Snippet: .. The full-length mouse RIG-I gene and its N-terminus, and the N-terminus of mouse MDA5 were amplified from a cDNA sample that isolated from RAW264.7 cells and cloned into pcDNA3.1/Flag-HA vector (Addgene), respectively. ..

    Article Title: Targeting GOF p53 and c-MYC through LZK Inhibition or Degradation Suppresses Head and Neck Tumor Growth
    Article Snippet: 293T cells were transiently transfected using Lipofectamine 2000 (Invitrogen), according to the manufacturer’s protocol, with OptiMEM (Gibco). .. A pcDNA3.1-FLAG-HA vector (Addgene #52535) was used as an empty vector control where required. ..

    Control:

    Article Title: Targeting GOF p53 and c-MYC through LZK Inhibition or Degradation Suppresses Head and Neck Tumor Growth
    Article Snippet: 293T cells were transiently transfected using Lipofectamine 2000 (Invitrogen), according to the manufacturer’s protocol, with OptiMEM (Gibco). .. A pcDNA3.1-FLAG-HA vector (Addgene #52535) was used as an empty vector control where required. ..



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